FREQUENCY OF CLAR AND JAK2 MUTATIONS IN SUDANESE CHRONIC MYELOID LEUKEMIA PATIENTS WITH PHILADELPHIA-POSITIVE DISEASE

Background: It is well-established that myeloproliferative diseases coexist with CLAR and JAK2. In Ph+ chronic myeloid leukemia (CML), only a few case reports indicate the existence of CLAR, JAK2V617F, JAK2 exon 12 mutations. Methods: This study examined CALR mutation profiles in Sudanese Chronic Myeloid Leukemia patients Philadelphia-positive patients. Blood samples were collected from 100 CML chromosomes. Results for JAK2V617F confirmed using TaqMan® Mutation Detection Assay, four common mutations on allele-specific PCR (AS-PCR) Sanger sequencing. Results: frameshift detected two (2%), found made up 4 (4%) total No significant relationships existed between age, WBC, RBC, Hb, HCT, or platelet parameters. Patients 12, have normal leukocyte counts lower values compared to triple-negative CML, but these differences are not statistically (p each 0.084, 0.173, 0.072). Conclusion: conceivable all coexist.